Week 12 : Gram staining

Day 1 : Receiving specimens at the bacteriology Section.

Samples include sputum, throat swab, blood culture, high vaginal swab, pus swab, stool, rectal swab, urine, ear swab, cord swab, urethral swab, eye swab and wound swab.

Upon entering patients' details into the record book, blood agar and macConkey agar were prepared.

Streaking on both agar was made.

Day 2. Plate checking. A single colony was taken for Gram staining.

Thin layer of cells from the single colony was made onto the slide and put on the staining station.

First, crystal violet solution was applied and stained for a minute.

Solution was discarded and rinsed thoroughly under running water.















Same procedure was repeated with Lugol's iodine, acetone and safranin O dye. Slides were dried with paper tower and checked under the microscope.

Numerous organism

Should a good single colony was taken, only one organism will be seen under the microscope.